Gilson 215 Manual do Utilizador descarregar pdf (Página 110)

D-2

Appendix D

Pipetting Techniques

Minimizing Cross-Contamination

Cross-contamination of samples is caused by

sample carryover on the probe tip or on its inner

walls. Several methods are available to minimize

cross-contamination.

• Use of the liquid level sensing capability will

reduce carryover by limiting immersion below

the liquid surface.

• Cleansing the probe tip with a flowing rinse

removes contaminants from the external

surfaces of the probe.

• Dispensing liquid through the probe into the

rinsing station will eliminate internal

contamination.

An alternate internal/external cleansing using a

non-flowing rinse is also possible. (Internal rinsing

may not be required at all if large diluent volumes

are dispensed immediately after dispensing of the

sample - a common practice in dilution

procedures.)

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Gilson 215 Manual do Utilizador Página 110