Gilson 215 Manual do Utilizador

Quad-Z 215 Liquid HandlerUser's GuideLT2545/©2003 Gilson, Inc. All rights reservedApril 2003

1-2Introduction 1DescriptionDescriptionThe Gilson Quad-Z 215 Liquid Handler is an XYZrobot that can automate any number of manualliquid handling proce

C-2Appendix CGSIOC CommandsGSIOC CommandsThere are two kinds of commands that you cansend over the GSIOC:• Buffered commands send instructions to thel

C-3Appendix CGSIOC Commands@ I Reads non-volatile memory (NV-RAM) at current address.Returns “aaa=xxxx” where:aaa - Value between 0 and 19 for word ty

C-4Appendix CGSIOC Commands9 B Clears the contact event FIFO and resets the contact inputevent timer.A I Reads home phases for X and Y motors. Returns

C-5Appendix CGSIOC CommandsExyz B Sets X, Y, and Z motor status:x - 0 for disable X motor.y - 0 for disable Y motor.z - 0 for disable or 1 for enable

C-6Appendix CGSIOC CommandsKsxxx B Sets liquid level sensing sensitivity where:s - selected probe (a, b, c, or d)xxx - Value from 0 to 255; 0 is the m

C-7Appendix CGSIOC CommandsN I Reads the liquid detector output. Returns “a,b,c,d” where:a - leftmost probe (probe A)b - probe Bc - probe Cd - probe D

C-8Appendix CGSIOC CommandsQ I Reads the XYZ travel range. Returns “axis=min/max”where:axis - X, Y or Z.min - Lowest position in tenths of millimeters

C-9Appendix CGSIOC Commandsvcs..s B Sends a GSIOC2 command directly to the Quad-Z where:c = the type of commands.ss = the command stringYou may send u

C-10Appendix CGSIOC CommandsZ I Reads probe Z-positions in integral units of 0.1 mm.Returns aaaa,bbbb,cccc,dddd where:aaaa is the Z-position of probe

D-1DPipetting TechniquesBy following these techniques, you’ll be able to maximize accuracy and precision, andminimize carryover, with your Quad-Z 215

1-3Introduction 1UnpackingUnpackingThe Quad-Z 215 Liquid Handler is delivered withall major components already assembled except forauxiliary parts suc

D-2Appendix DPipetting TechniquesMinimizing Cross-ContaminationCross-contamination of samples is caused bysample carryover on the probe tip or on its

D-3Appendix DPipetting TechniquesMinimizing Dilution EffectAn undesirable dilution sometimes occurs when anaspirated sample replaces rinse or diluent

D-4Appendix DPipetting TechniquesThe following example describes an instance whenthe above 8-step procedure is required. An operatorneeds to dispense

1-4Introduction 1UnpackingStandard EquipmentOnce the liquid handler and the accessoriescontainers have been unpacked, you should havethe following:Qua

1-5Introduction 1Customer ServiceCustomer ServiceGilson, Inc. and its worldwide network of authorizedrepresentatives provide customers with the follow

1-6Introduction 1Technical SpecificationsTechnical SpecificationsPlease be aware of the following before operatingthe liquid handler.Warning: Changes

1-7Introduction 1Technical Specifications

1-8Introduction 1Technical Specifications

2-12This section takes you through the steps for setting up your Quad-Z 215 Liquid Handler.Installation

2-2Installation 2Locator Plate InstallationLocator Plate InstallationThe locator plate serves two functions:• Positions the racks and accessories that

2-3Installation 2Armlock RemovalArmlock RemovalThe armlock on the liquid handler secures the Y-armduring shipment. You must remove the armlockprior to

2-4Installation 2Z-Arm InstallationZ-Arm InstallationFollow these steps to install the Z-arm:1 Using a Phillips screwdriver, loosen themounting screw

2-5Installation 2Z-Arm InstallationInstalling the Z-Arm Cable Support Rod1 Using the two Phillips screws, attach the cablesupport rod bracket in the h

2-6Installation 2Probe InstallationProbe InstallationThere are different probes available for use on theQuad-Z 215 Liquid Handler. Depending upon your

2-7Installation 2Plumbing ConnectionsPlumbing ConnectionsTransfer Tubing Installation1 Connect one end of each piece of transfertubing to the 444 Quad

2-8Installation 2Plumbing ConnectionsInstalling the Level Sensing CablesTo install the level sensing cable:1 Plug the right-angle connector on one end

2-9Installation 2Plumbing ConnectionsRinse Station and Drain WasteTubing InstallationYou’ll clean the probe using the rinse station. Toeliminate carry

2-10Installation 2Plumbing ConnectionsInstalling the rinse stationThe base of one rinse station is shipped alreadysecured to the locator plate and is

2-11Installation 2Rack SetupRack SetupThe Quad-Z 215 Liquid Handler is equipped tolocate Code 20-, 30-, 200-, and 500-series racks. SeeAppendix B for

2-12Installation 2Rack SetupCode 200-Series and Code 20-Seriesor Code 30-Series RacksTo use a combination of Code 200-series and Code20-series or Code

2-13Installation 2Rack SetupOnly Code 20-Series orCode 30-Series RacksIf all your racks are Code 20- or Code 30-series racksand you do not have the op

Table of ContentsDeclaration of Conformity1 IntroductionSafety Precautions ...1-1Descripti

2-14Installation 2Final Z-Arm AdjustmentFollow these steps to adjust the Z-arm to the properheight.1 Turn off power to the liquid handler.2 Locate the

2-15Installation 24 Loosen the mounting screw on the Z-armmounting bracket and slightly raise the Z-arm.5 Manually move the liquid handler’s arm so th

2-16Installation 2Electrical ConnectionsRear Panel1 Input/Output (I/O) ports2 Gilson Serial Input/Output Channel (GSIOC) port3 Keypad port (not used o

2-17Installation 2Input/Output PortsYou can use the input and output contacts foundon the rear panel of the liquid handler to controlperipheral device

2-18Installation 2Electrical ConnectionsMaking connectionsTo prepare and make connections with the 2-conductor cable:1 Cut the cable into pieces of ap

2-19Installation 2RS-232 PortThe RS-232 port is used to transfer informationbetween the liquid handler and a computer. For thelocation of the RS-232 p

2-20Installation 2GSIOC PortGilson systems feature a two-way communicationinterface between the computer and most Gilsonmodules. Communication occurs

2-21Installation 2Electrical ConnectionsUnit ID and Baud Rate/ModeSelectionUse the SW1 selector to choose a different unit IDand the SW2 to choose a d

2-22Installation 2Baud rate/modeAs a default, the baud rate/mode is set to 6,indicating that the liquid handler is set for a baudrate of 19200 and is

2-23Installation 2Electrical ConnectionsFusesYou received the liquid handler without any fusesinstalled. To install the fuses:1 Locate the accessory p

3 OperationFront Panel...3-2Start Button ...

2-24Installation 2Electrical ConnectionsPower Cord ConnectionLocate the appropriate power cord for your linevoltage. Discard the other power cord.Use

3-13OperationThe Quad-Z 215 Liquid Handler is controlled as follows:• Via a method running on a personal computer. You create the method using 735Samp

3-2Operation 3Front PanelFront PanelThe front panel of the liquid handler contains aStart button, Stop button, LED display, and powerindicator light.S

3-3Operation 3Start UpStart UpTo start the liquid handler:1 Make sure the liquid handler is connected to apower source.2 Turn on the liquid handler us

3-4Operation 3Running ProgramsRunning ProgramsThe liquid handler is controlled by programsexecuted from a personal computer.Executable (.EXE) programs

3-5Operation 3Configuring the Liquid HandlerConfiguring the Liquid HandlerThe liquid handler comes from the factory withits configuration set by Gilso

3-6Operation 3Configuring the Liquid HandlerAboutThe About tab allows you to indicate the unit ID ofthe instrument being configured so the 215 SetupUt

3-7Operation 3Pump TypeBecause the Quad-Z 215 Liquid Handler uses anexternal dilutor, Blank Front is selected and alloptions are inactive.Syringe Opti

3-8Operation 3Configuring the Liquid HandlerRinse SiteThe instrument stores the location of a rinse site inmemory. This allows the instrument to move

3-9Operation 3Liquid DetectorThe Liquid Detector tab allows you to adjust thedefault sensitivity for liquid detection.Note: Gilson control software (s

5 TroubleshootingError Messages ...5-2Mechanical...

3-10Operation 3Configuring the Liquid HandlerAdjust XYThe Adjust XY tab allows you to test whether theinstrument is properly adjusted and to make mino

3-11Operation 3model 819:X-coordinate: 544.0 mmY-coordinate: 3.8 mmIf the model 819 is not installed next to the rightsupport use the following coordi

3-12Operation 3Z HeightUse the Z Heights tab to identify the size of theinstalled Z-arm and the height at which the Z-armis clamped.The tower height o

3-13Operation 3Home PhaseUse the Home Phase tab to display the current X-and Y-phase of the instrument.Clicking Start causes the liquid handler to per

3-14Operation 3Configuring the Liquid HandlerSafety ContactThe instrument has provisions for connecting safetydevices that your application may requir

3-15Operation 3Sound and DisplayUse the options in the Sound and Display tab toadjust the brightness of the display, sound level,and tone.Clicking Bee

3-16Operation 3Testing the Liquid Handler’sContactsFollowing is a description of how to use the 215Contact Test Utility to toggle output contacts tode

4-14MaintenanceTo obtain optimum performance and maximum life from the Quad-Z 215 Liquid Handler,it is important to keep the instrument well-maintaine

4-2Maintenance 4Helpful HintsHelpful HintsIn order to keep your liquid handler at peakperformance, Gilson recommends that you do thefollowing:• Change

4-3Maintenance 4CleaningCleaningCleaning the Liquid HandlerThe liquid handler should be cleaned occasionallyusing a dry, clean cloth. Or, if necessary

4-4Maintenance 4CleaningCleaning methodsDepending on the samples or reagents that comeinto contact with the fluid path, you may need tovary your clean

4-5Maintenance 4CleaningAcidic and basic compounds - Follow thisprocedure if the fluid path is in contact with acidicand basic compounds:1 Prime the f

4-6Maintenance 4CleaningBiological fluids - Follow this procedure if thefluid path is in contact with biological fluids suchas blood products:1 Make a

4-7Maintenance 4LubricatingAlways switch the power to off when makingadjustments to (such as lubricating) the liquidhandler. The potential exists for

4-8Maintenance 46 Dispense another small bead of grease on theapplicator.7 Apply the grease to the visible and accessibleareas on the vertical rods an

4-9Maintenance 4Replacing PartsReplacing TubingIt is important to keep all tubing clean and free ofcrimps. Tubing that has become dirty, blocked orcri

4-10Maintenance 4Installing different type of probeTo install a replacement probe of a different typethan is currently installed, you may want to obta

4-11Maintenance 4Replacing a Level Sensing CableRemoving the level sensing cable1 Detach the transfer tubingfrom the top of the isolationprobe holder

4-12Maintenance 4Installing the new level sensing cable1 Locate the new level sensing cable suppliedwith the Z-arm.2 Pass the transfer tubing through

4-13Maintenance 4Replacing a FuseA blown fuse may indicate the existence of anotherproblem in the instrument. If the replacement fusesblow, don’t try

Application of Council Directives:89/336/EEC, 73/23/EECStandards to which Conformity is Declared:EN61326, EN61000-3-3, EN61000-3-2, EN61010-1Manufactu

4-14Maintenance 4Checking Position AlignmentThe 215 Setup Utility (Adjust XY tab), described inSection 3, allows you to test whether the liquidhandler

4-15Maintenance 4Transporting the Liquid HandlerTransporting the Liquid HandlerWhen moving the liquid handler to anotherlocation or when sending it ba

5-15Troubleshooting

5-2Troubleshooting 5Error MessagesError Description Solution15 NV-RAM checksum is invalid • Send the buffered ~9 GSIOC command toreset the NV-RAM and

5-3Troubleshooting 529 Z target more than maximum Z Send the immediate Q command using theGSIOC Utility Program to read the travel range.Correct the e

5-4Troubleshooting 5MechanicalProbe(s) no longer finding tube center• Probe(s) may be bent. Straighten or replace theprobe.• Incorrect tray file defin

5-5Troubleshooting 5ElectricalElectricalInput functions not operating• Make sure connections into terminal blockconnector are secure.• Make sure termi

5-6Troubleshooting 5ElectricalUnit not operational• Make sure power is turned on.• Check AC power cord connections.• Try different AC outlet.• Check f

5-7Troubleshooting 5Liquid Level DetectorLiquid Level DetectorLiquid level detector not detecting liquid level• Ensure that the level sensing cables a

5-8Troubleshooting 5Repair and Return PoliciesRepair and Return PoliciesBefore calling usGilson Customer Service personnel will be able toserve you mo

5-9Troubleshooting 5Repair and Return PoliciesRebuilt exchangeFor some units, rebuilt exchange components areavailable. Contact Gilson for details.Ret

A-1AReplacement Parts and AccessoriesProbes2507214 Non septum-piercing probe; constricted tip, capacitivelevel-sensing, stainless steel. Dimensions: 2

A-2Appendix AReplacement Parts and Accessories2507255 Non septum-piercing probe; beveled tip, capacitivelevel-sensing, stainless steel. Dimensions: 26

A-3Appendix AReplacement Parts and Accessories23077310 Waste bottle (2 liter) with lid and quick-connectfitting470343706 Tygon tubing (5/16" ID x

A-4Appendix AReplacement Parts and AccessoriesCables and I/O Accessories25061401 Level-sensing cable36083121 Serial cable, IBM PS/2-type, 25 to 25 pin

B-1BRacksThe Quad-Z 215 Liquid Handler can be configured with a variety of rack types and sizes.The following pages describe the racks that can be pur

B-2Appendix BRacksCode 20 rackFor 108 vesselsMaterial: polypropyleneVessels and maximum capacity: 10 x 100 mm tubes (4.5 mL)Part number: 150425Require

B-3Appendix BRacksCode 28 rackFor 108 vesselsMaterial: polypropyleneVessels and maximum capacity: 10 x 65 mm tubes (3 mL)10 x 75 mm tubes (3.5 mL)Part

1-11IntroductionSafety PrecautionsFor safe and correct use of this instrument, it is recommended that both operating andservice personnel follow the i

B-4Appendix BRacksCode 32 rackThermostated rack for 60 vesselsMaterial: aluminumVessels and maximum capacity: 13 x 65 mm tubes (6 mL)13 x 100 mm tubes

B-5Appendix BRacksCode 201 rackFor two microplates, two Eppendorf vials, andtwo 13 x 100 mm tubesMaterial: aluminumVessels and maximum capacity:96 wel

B-6Appendix BRacksCode 204 rackFor 27 scintillation tubesMaterial: aluminumVessels and maximum capacity: 28 x 57 mm (20 mL)Part number: 2504604Code 20

B-7Appendix BRacksCode 206 rackFor 96 vesselsMaterial: aluminumVessels and maximum capacity: 13 x 75 mm (7 mL)Part number: 2504606Code 207 rackFor 75

B-8Appendix BRacksCode 210 rackFor 75 vesselsMaterial: aluminumVessels and maximum capacity: 16 x 75 mm (11 mL)Part number: 2504610Code 211 rackFor 9

B-9Appendix BRacksCode 214 rackFor 96 vesselsMaterial: aluminumVessels and maximum capacity: 48 10.25 x 47 mm (2.5 mL)48 10.25 x 64 mm (44 mL)Part num

B-10Appendix BRacksCode 222 rackFor 27 conical bottom tubesMaterial: aluminumVessels and maximum capacity: 27 (50 mL)Part number: 2504622Code 228 rack

B-11Appendix BRacksPeltier RacksCode 242 Peltier rackFor 2 shallow, flat-bottom, 96-well microplatesMaterial: aluminumVessels and maximum capacity: 2

B-12Appendix BRacksCode 852 Peltier rackFor two Becton Dickinson Falcon 96-well, shallow-well assayplates. U-bottom styleMaterial: aluminumPart number

C-1CGSIOC CommandsThe Gilson Serial Input Output Channel (GSIOC) is an asynchronous serial communicationsinterface that enhances the power of your Gil